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Strand-Specific Postreplicative Processing of Mammalian Telomeres

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Science  28 Sep 2001:
Vol. 293, Issue 5539, pp. 2462-2465
DOI: 10.1126/science.1062560

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Abstract

Telomeres are specialized nucleoprotein structures that stabilize the ends of linear eukaryotic chromosomes. In mammalian cells, abrogation of telomeric repeat binding factor TRF2 or DNA-dependent protein kinase (DNA-PK) activity causes end-to-end chromosomal fusion, thus establishing an essential role for these proteins in telomere function. Here we show that TRF2-mediated end-capping occurs after telomere replication. The postreplicative requirement for TRF2 and DNA-PKcs, the catalytic subunit of DNA-PK, is confined to only half of the telomeres, namely, those that were produced by leading-strand DNA synthesis. These results demonstrate a crucial difference in postreplicative processing of telomeres that is linked to their mode of replication.

  • * To whom correspondence should be addressed. E-mail: egoodwin{at}telomere.lanl.gov

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