Research Article

Replication Dynamics of the Yeast Genome

Science  05 Oct 2001:
Vol. 294, Issue 5540, pp. 115-121
DOI: 10.1126/science.294.5540.115

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Abstract

Oligonucleotide microarrays were used to map the detailed topography of chromosome replication in the budding yeastSaccharomyces cerevisiae. The times of replication of thousands of sites across the genome were determined by hybridizing replicated and unreplicated DNAs, isolated at different times in S phase, to the microarrays. Origin activations take place continuously throughout S phase but with most firings near mid–S phase. Rates of replication fork movement vary greatly from region to region in the genome. The two ends of each of the 16 chromosomes are highly correlated in their times of replication. This microarray approach is readily applicable to other organisms, including humans.

  • * These authors contributed equally to this work.

  • To whom correspondence should be addressed. E-mail: raghu{at}u.washington.edu

  • Present address: Aventa Biosciences, 4757 Nexus Centre Drive, Suite 200, San Diego, CA 92121, USA.

  • § Present address: The Salk Institute for Biological Studies, Laboratory of Genetics, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.

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