Report

SNARE Function Analyzed in Synaptobrevin/VAMP Knockout Mice

Science  02 Nov 2001:
Vol. 294, Issue 5544, pp. 1117-1122
DOI: 10.1126/science.1064335

You are currently viewing the abstract.

View Full Text

Via your Institution

Log in through your institution

Log in through your institution


Abstract

SNAREs (soluble NSF-attachment protein receptors) are generally acknowledged as central components of membrane fusion reactions, but their precise function has remained enigmatic. Competing hypotheses suggest roles for SNAREs in mediating the specificity of fusion, catalyzing fusion, or actually executing fusion. We generated knockout mice lacking synaptobrevin/VAMP 2, the vesicular SNARE protein responsible for synaptic vesicle fusion in forebrain synapses, to make use of the exquisite temporal resolution of electrophysiology in measuring fusion. In the absence of synaptobrevin 2, spontaneous synaptic vesicle fusion and fusion induced by hypertonic sucrose were decreased ∼10-fold, but fast Ca2+-triggered fusion was decreased more than 100-fold. Thus, synaptobrevin 2 may function in catalyzing fusion reactions and stabilizing fusion intermediates but is not absolutely required for synaptic fusion.

  • * These authors contributed equally to this work.

  • To whom correspondence should be addressed. E-mail: Thomas.Sudhof{at}UTSouthwestern.edu, Ege.Kavalali{at}UTSouthwestern.edu

View Full Text

Cited By...