Defective Antigen Processing in GILT-Free Mice

See allHide authors and affiliations

Science  09 Nov 2001:
Vol. 294, Issue 5545, pp. 1361-1365
DOI: 10.1126/science.1065500

You are currently viewing the abstract.

View Full Text

Log in to view the full text

Log in through your institution

Log in through your institution


Processing of proteins for major histocompatibility complex (MHC) class II–restricted presentation to CD4-positive T lymphocytes occurs after they are internalized by antigen-presenting cells (APCs). Antigenic proteins frequently contain disulfide bonds, and their reduction in the endocytic pathway facilitates processing. In humans, a gamma interferon–inducible lysosomal thiol reductase (GILT) is constitutively present in late endocytic compartments of APCs. Here, we identified the mouse homolog of GILT and generated a GILT knockout mouse. GILT facilitated the processing and presentation to antigen-specific T cells of protein antigens containing disulfide bonds. The response to hen egg lysozyme, a model antigen with a compact structure containing four disulfide bonds, was examined in detail.

  • * Present address: Aventis Pasteur, Discovery Drive, Route 611, Swiftwater, PA 18370, USA.

  • Present address: Department of Immunology, University of Washington, 1959 N.E. Pacific Street, Seattle, WA 98195, USA.

  • Present address: Department of Immunology, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.

  • § To whom correspondence should be addressed. E-mail: peter.cresswell{at}

View Full Text