Report

Role of the Hrp Pilus in Type III Protein Secretion in Pseudomonas syringae

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Science  21 Dec 2001:
Vol. 294, Issue 5551, pp. 2556-2558
DOI: 10.1126/science.1066397

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Abstract

Bacterial surface appendages called pili and needle-like filaments are associated with protein and/or DNA transfer to recipient plant, human, or bacterial cells during pathogenesis or conjugation. Although it has long been suspected that pili function as a conduit for protein or DNA transfer, direct evidence has been lacking. The Hrp pilus ofPseudomonas syringae is assembled by the type III secretion system. We used an in situ immunogold labeling procedure to visualize the extrusion of an effector protein, AvrPto, from the tip of the Hrp pilus, providing direct evidence that a bacterial pilus can function as a conduit for protein delivery.

  • * To whom correspondence should be addressed. E-mail: hes{at}msu.edu

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