Cell Biology

GRASPing the Stack

See allHide authors and affiliations

Science  15 Feb 2002:
Vol. 295, Issue 5558, pp. 1197
DOI: 10.1126/science.295.5558.1197a

In mammalian cells, the Golgi complex consists of a set of stacked cisternal membranes on one side of the nucleus. Lane et al. describe how, in cells undergoing programmed cell death (apoptosis), this ribbon of Golgi membranes can fragment, producing clusters of tubules and vesicles, which is reminiscent of the Golgi breakdown observed when cells divide during mitosis. The fragmentation process required the activity of an apoptosis-specific protease, caspase-3, and proteolytic cleavage of the Golgi protein GRASP65 promoted fragmentation. Cells expressing a caspase-resistant GRASP65 were able to maintain the integrity of the Golgi complex during apoptosis, suggesting that this protein contributes to the structural underpinnings of the stacked cisternae. — SMH

J. Cell Biol. 156, 495 (2002).

Navigate This Article