Report

Requirement of the Activation-Induced Deaminase (AID) Gene for Immunoglobulin Gene Conversion

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Science  15 Feb 2002:
Vol. 295, Issue 5558, pp. 1301-1306
DOI: 10.1126/science.1067308

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Abstract

Three phenotypically distinct processes—somatic hypermutation, gene conversion, and switch recombination—remodel the functionally rearranged immunoglobulin (Ig) loci in B cells. Somatic hypermutation and switch recombination have recently been shown to depend on the activation-induced deaminase (AID) gene product. Here, we show that the disruption of the AID gene in the chicken B cell line DT40 completely blocks Ig gene conversion and that this block can be complemented by reintroduction of theAID complementary DNA. This demonstrates that theAID master gene controls all B cell–specific modifications of vertebrate Ig genes.

  • * To whom correspondence should be addressed. E-mail: buersted{at}hpi.uni-hamburg.de

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