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Array-Based Electrical Detection of DNA with Nanoparticle Probes

Science  22 Feb 2002:
Vol. 295, Issue 5559, pp. 1503-1506
DOI: 10.1126/science.1067003

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Abstract

A DNA array detection method is reported in which the binding of oligonucleotides functionalized with gold nanoparticles leads to conductivity changes associated with target-probe binding events. The binding events localize gold nanoparticles in an electrode gap; silver deposition facilitated by these nanoparticles bridges the gap and leads to readily measurable conductivity changes. An unusual salt concentration–dependent hybridization behavior associated with these nanoparticle probes was exploited to achieve selectivity without a thermal-stringency wash. Using this method, we have detected target DNA at concentrations as low as 500 femtomolar with a point mutation selectivity factor of ∼ 100,000:1.

  • * Present address: Department of Chemistry, University of Minnesota, Minneapolis, MN 55455, USA.

  • To whom correspondence should be addressed. E-mail: camirkin{at}chem.northwestern.edu

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