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Protein Nanoarrays Generated By Dip-Pen Nanolithography

Science  01 Mar 2002:
Vol. 295, Issue 5560, pp. 1702-1705
DOI: 10.1126/science.1067172

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Abstract

Dip-pen nanolithography was used to construct arrays of proteins with 100- to 350-nanometer features. These nanoarrays exhibit almost no detectable nonspecific binding of proteins to their passivated portions even in complex mixtures of proteins, and therefore provide the opportunity to study a variety of surface-mediated biological recognition processes. For example, reactions involving the protein features and antigens in complex solutions can be screened easily by atomic force microscopy. As further proof-of-concept, these arrays were used to study cellular adhesion at the submicrometer scale.

  • * To whom correspondence should be addressed. E-mail: camirkin{at}chem.northwestern.edu; for cell adhesion work: mmrksich{at}midway.uchicago.edu

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