Parallel Single-Cell Monitoring of Receptor-Triggered Membrane Translocation of a Calcium-Sensing Protein Module

+ See all authors and affiliations

Science  08 Mar 2002:
Vol. 295, Issue 5561, pp. 1910-1912
DOI: 10.1126/science.1065028

You are currently viewing the abstract.

View Full Text


Time courses of translocation of fluorescently conjugated proteins to the plasma membrane were simultaneously measured in thousands of individual rat basophilic leukemia cells. We found that the C2 domain—a calcium-sensing, lipid-binding protein module that is an essential regulator of protein kinase C and numerous other proteins—targeted proteins to the plasma membrane transiently if calcium was released from internal stores, and persistently in response to entry of extracellular calcium across the plasma membrane. The C2 domain translocation time courses of stimulated cells clustered into only two primary modes. Hence, the reversible recruitment of families of signaling proteins from one cellular compartment to another is a rapid bifurcation mechanism for inducing discrete states of cellular signaling networks.

  • * To whom correspondence should be addressed. E-mail: tobiasmeyer{at}

View Full Text