A Technique Whose Time Has Come

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Science  19 Apr 2002:
Vol. 296, Issue 5567, pp. 557-559
DOI: 10.1126/science.296.5567.557

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Real-time PCR is a molecular biological technique that allows the continuous monitoring of the amplification of a target DNA molecule throughout the PCR reaction. This essay gives an overview of the real-time PCR methodology, the application of this technology to the quantification levels of nucleic acids in biological samples, and the impact of this approach on genomics and medicine.