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An LRR Receptor Kinase Involved in Perception of a Peptide Plant Hormone, Phytosulfokine

Science  24 May 2002:
Vol. 296, Issue 5572, pp. 1470-1472
DOI: 10.1126/science.1069607

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Abstract

The sulfated peptide phytosulfokine (PSK) is an intercellular signal that plays a key role in cellular dedifferentiation and proliferation in plants. Using ligand-based affinity chromatography, we purified a 120-kilodalton membrane protein, specifically interacting with PSK, from carrot microsomal fractions. The corresponding complementary DNA encodes a 1021–amino acid receptor kinase that contains extracellular leucine-rich repeats, a single transmembrane domain, and a cytoplasmic kinase domain. Overexpression of this receptor kinase in carrot cells caused enhanced callus growth in response to PSK and a substantial increase in the number of tritium-labeled PSK binding sites, suggesting that PSK and this receptor kinase act as a ligand-receptor pair.

  • * To whom correspondence should be addressed. E-mail: matsu{at}agr.nagoya-u.ac.jp

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