Report

Regulation of MAPK Function by Direct Interaction with the Mating-Specific Gα in Yeast

+ See all authors and affiliations

Science  24 May 2002:
Vol. 296, Issue 5572, pp. 1483-1486
DOI: 10.1126/science.1070540

You are currently viewing the abstract.

View Full Text

Abstract

The mating response of the budding yeast Saccharomyces cerevisiae is mediated by a prototypical heterotrimeric GTP-binding protein (G protein) and mitogen-activated protein kinase (MAPK) cascade. Although signal transmission by such pathways has been modeled in detail, postreceptor down-regulation is less well understood. The pheromone-responsive G protein α subunit (Gα) of yeast down-regulates the mating signal, but its targets are unknown. We have found that Gα binds directly to the mating-specific MAPK in yeast cells responding to pheromone. This interaction contributes both to modulation of the mating signal and to the chemotropic response, and it demonstrates direct communication between the top and bottom of a Gα-MAPK pathway.

  • * To whom correspondence should be addressed. E-mail: dstone{at}uic.edu

View Full Text