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Two-Photon Imaging of Lymphocyte Motility and Antigen Response in Intact Lymph Node

Science  07 Jun 2002:
Vol. 296, Issue 5574, pp. 1869-1873
DOI: 10.1126/science.1070051

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Abstract

Lymphocyte motility is vital for trafficking within lymphoid organs and for initiating contact with antigen-presenting cells. Visualization of these processes has previously been limited to in vitro systems. We describe the use of two-photon laser microscopy to image the dynamic behavior of individual living lymphocytes deep within intact lymph nodes. In their native environment, T cells achieved peak velocities of more than 25 micrometers per minute, displaying a motility coefficient that is five to six times that of B cells. Antigenic challenge changed T cell trajectories from random walks to “swarms” and stable clusters. Real-time two-photon imaging reveals lymphocyte behaviors that are fundamental to the initiation of the immune response.

  • * These authors contributed equally to this work.

  • To whom correspondence should be addressed. E-mail: mcahalan{at}uci.edu

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