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Structure of an HIF-1α-pVHL Complex: Hydroxyproline Recognition in Signaling

Science  07 Jun 2002:
Vol. 296, Issue 5574, pp. 1886-1889
DOI: 10.1126/science.1073440

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Abstract

The ubiquitination of the hypoxia-inducible factor (HIF) by the von Hippel–Lindau tumor suppressor (pVHL) plays a central role in the cellular response to changes in oxygen availability. pVHL binds to HIF only when a conserved proline in HIF is hydroxylated, a modification that is oxygen-dependent. The 1.85 angstrom structure of a 20-residue HIF-1α peptide–pVHL–ElonginB–ElonginC complex shows that HIF-1α binds to pVHL in an extended β strand–like conformation. The hydroxyproline inserts into a gap in the pVHL hydrophobic core, at a site that is a hotspot for tumorigenic mutations, with its 4-hydroxyl group recognized by buried serine and histidine residues. Although the β sheet–like interactions contribute to the stability of the complex, the hydroxyproline contacts are central to the strict specificity characteristic of signaling.

  • * To whom correspondence should be addressed. E-mail: nikola{at}xray2.mskcc.org.

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