Report

Channelrhodopsin-1: A Light-Gated Proton Channel in Green Algae

Science  28 Jun 2002:
Vol. 296, Issue 5577, pp. 2395-2398
DOI: 10.1126/science.1072068

You are currently viewing the abstract.

View Full Text
As a service to the community, AAAS/Science has made this article free with registration.

Abstract

Phototaxis and photophobic responses of green algae are mediated by rhodopsins with microbial-type chromophores. We report a complementary DNA sequence in the green alga Chlamydomonas reinhardtiithat encodes a microbial opsin-related protein, which we term Channelopsin-1. The hydrophobic core region of the protein shows homology to the light-activated proton pump bacteriorhodopsin. Expression of Channelopsin-1, or only the hydrophobic core, inXenopus laevis oocytes in the presence of all-trans retinal produces a light-gated conductance that shows characteristics of a channel selectively permeable for protons. We suggest that Channelrhodopsins are involved in phototaxis of green algae.

  • * To whom correspondence should be addressed. E-mail: nagel{at}mpibp-frankfurt.mpg.de

View Full Text

Cited By...