Report

Channelrhodopsin-1: A Light-Gated Proton Channel in Green Algae

Science  28 Jun 2002:
Vol. 296, Issue 5577, pp. 2395-2398
DOI: 10.1126/science.1072068

You are currently viewing the abstract.

View Full Text

Via your Institution

Log in through your institution

Log in through your institution


Abstract

Phototaxis and photophobic responses of green algae are mediated by rhodopsins with microbial-type chromophores. We report a complementary DNA sequence in the green alga Chlamydomonas reinhardtiithat encodes a microbial opsin-related protein, which we term Channelopsin-1. The hydrophobic core region of the protein shows homology to the light-activated proton pump bacteriorhodopsin. Expression of Channelopsin-1, or only the hydrophobic core, inXenopus laevis oocytes in the presence of all-trans retinal produces a light-gated conductance that shows characteristics of a channel selectively permeable for protons. We suggest that Channelrhodopsins are involved in phototaxis of green algae.

  • * To whom correspondence should be addressed. E-mail: nagel{at}mpibp-frankfurt.mpg.de

View Full Text

Cited By...