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Est1p As a Cell Cycle-Regulated Activator of Telomere-Bound Telomerase

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Science  09 Aug 2002:
Vol. 297, Issue 5583, pp. 1023-1026
DOI: 10.1126/science.1074968

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Abstract

In Saccharomyces cerevisiae, the telomerase components Est2p, TLC1 RNA, Est1p, and Est3p are thought to form a complex that acts late during chromosome replication (S phase) upon recruitment by Cdc13p, a telomeric DNA binding protein. Consistent with this model, we show that Est1p, Est2p, and Cdc13p are telomere-associated at this time. However, Est2p, but not Est1p, also binds telomeres before late S phase. The cdc13-2 allele has been proposed to be defective in recruitment, yet Est1p and Est2p telomere association persists in cdc13-2 cells. These findings suggest a model in which Est1p binds telomeres late in S phase and interacts with Cdc13p to convert inactive, telomere-bound Est2p to an active form.

  • * Present address: Department of Microbiology, College of Medicine, National Taiwan University, Taiwan.

  • To whom correspondence should be addressed. E-mail: vzakian{at}molbio.princeton.edu

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