TOOL: DNA Primers to Go

Science  14 Mar 2003:
Vol. 299, Issue 5613, pp. 1637
DOI: 10.1126/science.299.5613.1637d

Our genes are messy, with stretches that code for proteins interrupted by extraneous DNA. This jumbled construction complicates the job of designing primers, the short sequences that serve as a starting point for the gene-copying technique known as PCR. ExonPrimer, a tool designed by Tim Strom of the Institute for Human Genetics in Neuherberg, Germany, helps researchers clear this hurdle. Users enter a gene's raw chromosome sequence and the sequence of its cDNA, a DNA version of its messenger RNA. Out pops a set of primers that can be used to replicate the functional part of the gene.

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