Research Article

Myosin V Walks Hand-Over-Hand: Single Fluorophore Imaging with 1.5-nm Localization

Science  27 Jun 2003:
Vol. 300, Issue 5628, pp. 2061-2065
DOI: 10.1126/science.1084398

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Abstract

Myosin V is a dimeric molecular motor that moves processively on actin, with the center of mass moving ∼37 nanometers for each adenosine triphosphate hydrolyzed. We have labeled myosin V with a single fluorophore at different positions in the light-chain domain and measured the step size with a standard deviation of <1.5 nanometers, with 0.5-second temporal resolution, and observation times of minutes. The step size alternates between 37 + 2x nm and 37 – 2x, where x is the distance along the direction of motion between the dye and the midpoint between the two heads. These results strongly support a hand-over-hand model of motility, not an inchworm model.

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