ANALYTICAL CHEMISTRY

Losing Data in the Ozone

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Science  08 Aug 2003:
Vol. 301, Issue 5634, pp. 737
DOI: 10.1126/science.301.5634.737b

Data collected from DNA microarrays can vary quite a bit, and Fare et al. have tracked down one potential problem that can produce uneven deterioration of the red and green fluorescent signals. During posthybridization stringency washes, cyanine dyes are exposed to and can be degraded by low levels of ozone exposure in the lab. The fluorescence of some dyes diminished at ozone levels as low 10 parts per billion (ppb), whereas others were stable at 100 ppb (just below the level at which ozone alerts would be issued for outside air quality). The loss of signal resulted in single spots appearing as a red center with a green surround. The authors suggest that air filtering within labs, which can keep ozone levels below 2 ppb, should suffice to minimize variation caused by this effect. — PDS

Anal. Chem. 10.1021/ac034241b (2003).

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