Report

Nuclear Membrane Proteins with Potential Disease Links Found by Subtractive Proteomics

See allHide authors and affiliations

Science  05 Sep 2003:
Vol. 301, Issue 5638, pp. 1380-1382
DOI: 10.1126/science.1088176

You are currently viewing the abstract.

View Full Text

Abstract

To comprehensively identify integral membrane proteins of the nuclear envelope (NE), we prepared separately NEs and organelles known to cofractionate with them from liver. Proteins detected by multidimensional protein identification technology in the cofractionating organelles were subtracted from the NE data set. In addition to all 13 known NE integral proteins, 67 uncharacterized open reading frames with predicted membrane-spanning regions were identified. All of the eight proteins tested targeted to the NE, indicating that there are substantially more integral proteins of the NE than previously thought. Furthermore, 23 of these mapped within chromosome regions linked to a variety of dystrophies.

    View Full Text