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Diffusion Dynamics of Glycine Receptors Revealed by Single-Quantum Dot Tracking

Science  17 Oct 2003:
Vol. 302, Issue 5644, pp. 442-445
DOI: 10.1126/science.1088525

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Abstract

Semiconductor quantum dots (QDs) are nanometer-sized fluorescent probes suitable for advanced biological imaging. We used QDs to track individual glycine receptors (GlyRs) and analyze their lateral dynamics in the neuronal membrane of living cells for periods ranging from milliseconds to minutes. We characterized multiple diffusion domains in relation to the synaptic, perisynaptic, or extrasynaptic GlyR localization. The entry of GlyRs into the synapse by diffusion was observed and further confirmed by electron microscopy imaging of QD-tagged receptors.

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