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p53- and Drug-Induced Apoptotic Responses Mediated by BH3-Only Proteins Puma and Noxa

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Science  07 Nov 2003:
Vol. 302, Issue 5647, pp. 1036-1038
DOI: 10.1126/science.1090072

Abstract

Apoptosis provoked by DNA damage requires the p53 tumor suppressor, but which of the many p53-regulated genes are required has remained unknown. Two genes induced by this transcription factor, noxa and puma (bbc3), stand out, because they encode BH3-only proteins, proapoptotic members of the Bcl-2 family required to initiate apoptosis. In mice with either noxa or puma disrupted, we observed decreased DNA damage–induced apoptosis in fibroblasts, although only loss of Puma protected lymphocytes from cell death. Puma deficiency also protected cells against diverse p53-independent cytotoxic insults, including cytokine deprivation and exposure to glucocorticoids, the kinase inhibitor staurosporine, or phorbol ester. Hence, Puma and Noxa are critical mediators of the apoptotic responses induced by p53 and other agents.

DNA damage can cause cell cycle arrest or apoptosis, and both responses contribute to tumor suppression by p53 (1, 2). After DNA damage, p53 imposes arrest in the G1 phase of the cell cycle by inducing expression of the cyclin-dependent kinase inhibitor p21Cip1, but how p53 triggers apoptosis is unresolved (1, 2). Because more than 16 p53 target genes have been proposed to mediate apoptosis, it has been unclear whether any single target is critical (1, 2). Noxa (3) and Puma (Bbc3) (46), however, merit particular attention, because BH3-only proteins [the proteins related to the Bcl-2 family only by the BH3 (Bcl-2 homology region 3) interaction domain] are essential triggers for the evolutionarily conserved path to apoptosis. Their binding to the antiapoptotic protein Bcl-2 or its close relatives launches the program, which proceeds through Bax-like family members to the proteases (caspases) that dismantle the cell (79). How cytotoxic drugs that kill in a p53-independent manner (for example, glucocorticoids) initiate apoptosis is also unclear, but because Bcl-2 overexpression inhibits this response (10, 11), BH3-only proteins are probably critical.

Noxa-deficient mice were generated from embryonic stem (ES) cells from which noxa exons 2 and 3, which encode its two BH3 regions, had been removed (fig. S1). Correct targeting of the gene was verified (Fig. 1A), and the absence of noxa mRNA was confirmed both by reverse transcription–polymerase chain reaction (RT-PCR) analysis on irradiated thymocytes from noxa–/– mice (Fig. 1B) and by Northern blots on several tissues (Fig. 1C). Noxa does not seem to be required for normal development or physiology, because the nullizygous mice were born at the expected Mendelian frequency from noxa+/– matings and became healthy adults. Their appearance, body weight, and organ weights were normal, as were their cellularity and the composition of hemopoietic organs (12).

Fig. 1.

Characterization of Noxa-deficient mice. (A) Southern blot (top) and PCR analysis (bottom) of genomic DNA from wild-type (+/+), noxa+/–, and noxa–/– mice. (B) Thymocytes from wild-type and noxa–/– mice were γ-irradiated [5 gray(Gy)] and RNA isolated after 6 hours. RT-PCR was followed by Southern blotting with an internal oligonucleotide probe specific for noxa cDNA. RT-PCR with primers for gapdh and probing with an internal gapdh probe was used as a loading control. (H2O: RT-PCR with primers for noxa or gapdh and probing with internal noxa or gapdh probes in tubes to which no cDNA was added.) (C) Northern blot analysis (top) of polyadenalated [poly (A)+] RNA isolated from tissues of wild-type and noxa–/– mice (top) and a picture of ethidium bromide–stained gel (bottom) used as a loading control. S, Svedberg. (D) Resistance of Noxa-deficient fibroblasts to DNA damage–induced apoptosis. Wild-type, noxa–/–, or p53–/– MEFs were left untreated or treated with etoposide (100 μg/mL). Alternatively, MEFs infected with a retrovirus that expresses E1A were treated with etoposide (100 μg/mL). Untreated MEFs expressing E1A had >90% viability at these times. Cell survival was quantified bystaining with AnnexinV-FITC (fluorescein isothiocyanate) plus propidium iodide (PI) and by flow cytometric analysis. Data represent means ± SD of cells from three or four mice of each genotype.

The role of Noxa in stress-induced apoptosis was investigated in thymocytes (fig. S2), pre-B cells, and mature B and T cells (12), as well as in primary mouse embryo fibroblasts (MEFs) (Fig. 1D). We tested both p53-dependent stimuli, such as the topoisomerase inhibitor etoposide and γ radiation (13, 14), and p53-independent ones, such as cytokine withdrawal, the glucocorticoid dexamethasone, ionomycin (which causes calcium flux), and the phorbol ester 12-myristate 13-acetate (PMA). Noxa loss did not protect against these death stimuli in thymocytes (fig. S2), nor in the other lymphocytes (12). In contrast, noxa–/– MEFs exhibited modest but significant resistance (P < 0.02, two-tailed t test) to etoposide-induced apoptosis (Fig. 1D). In MEFs rendered sensitive to p53-mediated apoptosis by the adenovirus oncoprotein E1A (15), loss of Noxa was also protective against etoposide (Fig. 1D).

Puma-deficient mice were generated similarly from ES cells that lacked puma exons 2 and 3, which encompass the start site and the BH3 region (fig. S3). Correct targeting was demonstrated (Fig. 2, A and B), and the absence of puma mRNA was verified in cells from puma–/– mice by RT-PCR (Fig. 2C) and by Northern blot analyses (Fig. 2D). Puma also seems dispensable for normal development and health, because Puma-deficient mice were born at the expected frequency, had a normal appearance and normal body and organ weights, and exhibited normal cellularity and composition of hemopoietic organs (12).

Fig. 2.

Molecular characterization of Puma-deficient mice. (A) Southern blot analysis on genomic DNA from Puma-deficient and littermate control mice. (B) PCR analysis with exon 2-specific primers on tail DNA from Puma-deficient and littermate control mice. The identity of the PCR product (shown by ethidium bromide staining, top) was confirmed by Southern blotting with an exon 2-specific internal oligonucleotide as a probe (bottom). (C) RT-PCR analysis on cDNAs generated from total RNA of Puma-deficient and littermate control mice (top). RT-PCR with primers specific for hprt was used as a loading control (shown byethidium bromide staining, bottom). (D) Northern blot analysis (top) of poly (A)+ RNA (4 μg) from irradiated (5 Gy) or untreated puma–/– or wild type thymocytes or spleen cells from cultured for 5 hours confirms loss of puma mRNA in the mutant mice. Full-length mouse puma cDNA was used as a probe. The filter was stripped and reprobed with a glyceraldehyde phosphate dehydrogenase (GAPDH) probe (bottom) to demonstrate the presence of RNA in all lanes.

The role of Puma in stress-induced apoptosis was investigated in thymocytes (Fig. 3, A and B), other lymphocytes (12), and MEFs (Fig. 3C). The puma–/– thymocytes were markedly resistant to genotoxic damage (by etoposide and γ radiation), albeit less so than p53–/– thymocytes (Fig. 3A and fig. S2) (13, 14). The puma–/– pre–B and mature B and T cells were also very refractory to DNA damage (12). Among p53-independent insults, the puma–/– thymocytes remained sensitive to ionomycin but were refractory to several others, specifically, cytokine deprivation, dexamethasone, the kinase inhibitor staurosporine, and most markedly, PMA (Fig. 3, A and B). In nontransformed and E1A-expressing MEFs, loss of Puma also markedly enhanced survival after serum withdrawal or etoposide treatment (Fig. 3C).

Fig. 3.

Compromised p53-dependent and p53-independent cell death responses in cells from mice that lack Puma. (A) Thymocytes from Puma-deficient mice, littermate control mice, a p53/– mouse, or mice that express a bcl-2 transgene controlled by the panhemopoietic vav gene promoter (24) were cultured for the indicated times after exposure to γ radiation. Cell viability was analyzed as in Fig. 1. Data points represent means ± SE of three independent experiments and three to five animals per genotype, except for the p53–/– control (n = 1). (B) Immature (CD4+8+) thymocytes from 6- to 12-week-old mice were sorted and cultured in normal medium in the absence or presence of the indicated cell death stimuli. Viabilitywas assessed after the indicated times by PI exclusion and flow cytometry. Data points represent means ± SE of five independent experiments and six to eight animals per genotype. (C) MEFs retrovirallytransduced with the E1A oncogene and selected in puromycin were cultured in simple medium with or without serum or were exposed to etoposide. Cell viabilitywas analyzed as in Fig. 1. Data points represent means ± SE of three independent experiments.

Our results demonstrate that Puma plays a major role in DNA damage–induced apoptosis and Noxa a more restricted role [see also (16)] (Fig. 4). In accord with this conclusion, p53-mediated apoptosis has been inhibited in a human colon carcinoma cell line by deletion of PUMA (17) and in mouse fibroblasts by reduction in Noxa expression through RNA interference (18). Transcription of both these genes is up-regulated by p53 (36) (Figs. 1, B and C, and 2D). Together with the requirement for protein synthesis in p53-mediated apoptosis of thymocytes (19) and the failure of a mutant p53 unable to affect transcriptioin to induce apoptosis (20, 21), our results strongly suggest that p53 promotes apoptosis mainly through transcriptional activation rather than through other proposed mechanisms (2). Puma and Noxa presumably contribute to all the p53-mediated apoptotic signals linked to tumorigenesis, including those from deregulated oncogenes (e.g., myc and ras), telomere erosion, and hypoxia (Fig. 4) (2). Although no mice lacking Puma or Noxa have yet developed tumors, assessing their tumorigenic role will require cohorts of aged mice.

Fig. 4.

Model for the apoptotic responses of cells to cytotoxic drugs, radiation, and cytokine withdrawal. The BH3-only proteins Puma, Noxa, and Bim mediate both stress responses dependent on p53 (2) and those independent of p53. These responses are activated both by imposed forms of stress (cytokine deprivation, cytotoxic drugs, and radiation) and by the stress signals that arise during tumorigenesis from activated oncogenes, telomere erosion, and hypoxia. Activation of the ATM kinase or the Arf protein inhibits the Mdm2 protein, allowing the level of p53 protein to increase. P53 then induces transcription of the genes for p21, Puma, and Noxa, as well as other targets of uncertain function (dashed box). Engagement of Bcl-2 (and its close relatives) by the BH3-onlyproteins then triggers activation of Bax and Bak and subsequent apoptotic events (9). STS, staurosporine; dex, dexamethasone.

Loss of Puma or Noxa provided less protection against DNA damage–induced apoptosis than p53 deficiency (13, 14) (Fig. 3A and fig. S2) or Bcl-2 overexpression (10, 22) (Fig. 3A). We therefore suggest that this death program (Fig. 4) relies on the redundant action of more than one BH3-only protein, including both Puma and Noxa. Although the BH3-only protein Bim is not a p53 target (12), it must also contribute indirectly (Fig. 4), because lymphocytes lacking Bim are somewhat refractory to γ radiation (7, 12). The roles, if any, of other proposed mediators of p53-induced apoptosis (Fig. 4) (2) remain to be established by gene targeting.

Puma also proved to be required for apoptosis induced by diverse p53-independent death stimuli, including cytokine withdrawal, staurosporine, PMA, and the chemotherapy agent dexamethasone (Figs. 3 and 4). The increased puma transcription provoked by dexamethasone (6) indicates that puma can be induced by the glucocorticoid receptor, but the pathway by which the other cytotoxic stimuli induce puma remains to be determined. In lymphocytes, Puma, Bim (7, 23), or both appear to have a critical role in the majority of cytotoxic responses (Fig. 4). Thus, BH3-only proteins have both overlapping and specialized roles as death initiators.

Supporting Online Material

www.sciencemag.org/cgi/content/full/1090072/DC1

Materials and Methods

Figs. S1 to S3

References and Notes

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