BIOTECHNOLOGY: Splicing in Diversity

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Science  21 Nov 2003:
Vol. 302, Issue 5649, pp. 1295d
DOI: 10.1126/science.302.5649.1295d

The antigen-binding end of an antibody molecule and green fluorescent protein (GEP) both contain β-strands that are connected by exposed loops. Zeytun et al. have taken advantage of this commonality and engineered libraries of “fluorobodies” by inserting antigen-binding sequences (the complementarity-determining regions) within four of the GFP loops. They isolated fluorobodies that bound specifically to several proteins (ubiquitin, tubulin, and myoglobin) with submicromolar affinities and were used successfully in immunofluorescence microscopy, immunoblots, or flow cytometry. — GJC

Nature Biotechnol. 10.1038/nbt911 (2003).

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