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Yeast Life-Span Extension by Calorie Restriction Is Independent of NAD Fluctuation

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Science  19 Dec 2003:
Vol. 302, Issue 5653, pp. 2124-2126
DOI: 10.1126/science.1088697

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Abstract

Calorie restriction (CR) slows aging in numerous species. In the yeast Saccharomyces cerevisiae, this effect requires Sir2, a conserved NAD+-dependent deacetylase. We report that CR reduces nuclear NAD+ levels in vivo. Moreover, the activity of Sir2 and its human homologue SIRT1 are not affected by physiological alterations in the NAD+:NADH ratio. These data implicate alternate mechanisms of Sir2 regulation by CR.

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