High-Throughput Mapping of a Dynamic Signaling Network in Mammalian Cells

+ See all authors and affiliations

Science  11 Mar 2005:
Vol. 307, Issue 5715, pp. 1621-1625
DOI: 10.1126/science.1105776

You are currently viewing the abstract.

View Full Text


Signaling pathways transmit information through protein interaction networks that are dynamically regulated by complex extracellular cues. We developed LUMIER (for luminescence-based mammalian interactome mapping), an automated high-throughput technology, to map protein-protein interaction networks systematically in mammalian cells and applied it to the transforming growth factor–β (TGFβ) pathway. Analysis using self-organizing maps and k-means clustering identified links of the TGFβ pathway to the p21-activated kinase (PAK) network, to the polarity complex, and to Occludin, a structural component of tight junctions. We show that Occludin regulates TGFβ type I receptor localization for efficient TGFβ-dependent dissolution of tight junctions during epithelial-to-mesenchymal transitions.

View Full Text