Cell Biology

Complex Cellularization

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Science  03 Jun 2005:
Vol. 308, Issue 5727, pp. 1379
DOI: 10.1126/science.308.5727.1379d

Early insect development involves multiple nuclear divisions within a single cytoplasm to form syncytial embryos. The syncytium is divided into separate cells (each with a single nucleus) in a process termed cellularization, which involves the generation of membrane furrows between adjacent nuclei and produces a polarized cortical cell layer. The formation of the cleavage furrow requires concerted delivery (from the Golgi complex) of membrane components to the growing furrow. This delivery increases the cell surface area by 20-fold and is directed by the microtubule network. Papoulas et al. followed the apically directed movement of Golgi complexes toward the sites of furrow formation, which depended on the activity of the microtubule-based molecular motor dynein. The Golgi membranes themselves interacted with dynein and other motility factors via a peripheral Golgi membrane protein of the golgin family, Lava lamp. These interactions were disrupted and cellularization blocked when domains from the Lava lamp protein that bound to dynein or the motility factors were injected into living embryos. — SMH

Nat. Cell Biol. 10.1038/ncb1264 (2005).

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