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Response to Comment on "Nervy Links Protein Kinase A to Plexin-Mediated Semaphorin Repulsion"

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Science  22 Jul 2005:
Vol. 309, Issue 5734, pp. 558
DOI: 10.1126/science.1109259
  • Fig. 1.

    Nervy (Nvy) antisense probes reveal Nvy transcript in axons of wild-type embryos but not Nvy mutant embryos. Filleted preparations of stage 16 Drosophila embryos (anterior, left). (A) In situ hybridization with antisense Nvy shows Nvy transcript within neurons and their axons (arrows), some of which can be seen here emanating from a neuronal cell body (ventral unpaired median neuron, white arrow). (B) In situ hybridization with antisense Nvy in a Nvy [PDFKG1 (2)] mutant embryo shows no evidence of Nvy in neuronal cell bodies or axons. (C) In situ hybridization with sense Nvy probe shows no specific staining of neurons or axons. Methods as in (2, 3).

  • Fig. 2.

    Immunohistochemistry with ETO/Nvy antisera shows that in wild-type, but not in the Nvy mutant, the Drosophila embryonic nervous system can be stained. Stage 16/17 embryos immunostained with anti-ETO/Nvy Ab-1 (Lot D16428-1, EMD Biosciences) in 2003 and reported in (2, 3) observed at low (A and B), intermediate (C and D), and high (E and F) power. The nervous system (arrowheads) immunostains in wild-type embryos [(A), (C), and (E)] but not in Nvy [PDFKG1 (2)] mutant embryos [(B), (D), and (F)]. Select immunostained axonal processes are indicated in (C) [arrows in the inset, emanating from a neuron (arrowhead)] and in (E) (arrows indicating multiple commissural axons). Axons were not immunostained in the Nvy mutant. Arrows in (F) indicate position of anterior and posterior commissures, visualized by Nomarski optics. Anterior is to the left. A, anterior commissure; P, posterior commissure; L, longitudinal connectives; asterisks, motor nerve roots. Methods as in (2, 3).

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  • Full Text Response to Comment on "Nervy Links Protein Kinase A to Plexin-Mediated Semaphorin Repulsion"
    J. R. Terman and A. L. Kolodkin

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