Research Article

Structure of a Synaptic γδ Resolvase Tetramer Covalently Linked to Two Cleaved DNAs

Science  19 Aug 2005:
Vol. 309, Issue 5738, pp. 1210-1215
DOI: 10.1126/science.1112064

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Abstract

The structure of a synaptic intermediate of the site-specific recombinase γδ resolvase covalently linked through Ser10 to two cleaved duplex DNAs has been determined at 3.4 angstrom resolution. This resolvase, activated for recombination by mutations, forms a tetramer whose structure is substantially changed from that of a presynaptic complex between dimeric resolvase and the cleavage site DNA. Because the two cleaved DNA duplexes that are to be recombined lie on opposite sides of the core tetramer, large movements of both protein and DNA are required to achieve strand exchange. The two dimers linked to the DNAs that are to be recombined are held together by a flat interface. This may allow a 180° rotation of one dimer relative to the other in order to reposition the DNA duplexes for strand exchange.

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