Science  07 Oct 2005:
Vol. 310, Issue 5745, pp. 49b
DOI: 10.1126/science.310.5745.49b

I wish to retract the Research Article “Hairpin RNAs and retrotransposon LTRs effect RNAi and chromatin-based gene silencing” (1). My laboratory is unable to reproduce the observations reported in Figs. 1 and 2 because we do not possess the plasmid pshura4SE-280 plasmid or fission yeast strains that contain it. In addition, attempts to reproduce these observations with other ura4-hairpin constructs have failed. I therefore retract the conclusion that expression of a hairpin RNA homologous to ura4 RNA results in the production of siRNA that bring about methylation of histone H3 on lysine 9 and recruitment of Swi6 to the ura4+gene in Schizosaccharomyces pombe. In addition, the data (Figs. 3, 4, and 5) indicating that long terminal repeats (LTRs) are enriched in H3K9me2 and Swi6 chromatin and that the expression of genes lying close to LTRs in cells lacking Ago1, Dcr1, Rdp1, Clr4 or Swi6 is increased are not reproducible by us or others. I therefore retract the conclusion that LTRs in S. pombeare packaged in RNAi-dependent heterochromatin and that these LTRs are involved in the regulation of nearby genes. I deeply regret any inconvenience that publication of these data has caused for others. The first author of the original paper, Vera Schramke, declined to be an author of this retraction.


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