Cell Biology

Recruitment to an Organization

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Science  24 Feb 2006:
Vol. 311, Issue 5764, pp. 1075
DOI: 10.1126/science.311.5764.1075a

Eukaryotic cells contain a dynamic array of microtubules (tubulin polymers), which play diverse roles in interphase but are dramatically rearranged into a spindle during mitosis to promote chromosome segregation. The centrosome, which is composed of a pair of centrioles and associated material, is a key organizer of microtubules and contains the γ isoform of tubulin. In mammalian cells, γ-tubulin is found in a ring-like complex together with other proteins, and Haren et al. characterize NEDD1, a protein of the centrosome that is associated with γ-tubulin ring structures. NEDD1 is not required for γ-tubulin ring complex assembly; in its absence, the complex is not correctly recruited to the centrosomes although NEDD1 is targeted to the centrosome even in the absence of γ-tubulin. Depletion of NEDD1 causes centrosomal defects and compromises the quality of the mitotic spindle and microtubule organization in interphase cells. Interfering with the NEDD1-γ-tubulin interaction blocks centriole duplication. Thus, it appears that NEDD1 mediates the interaction between γ-tubulin and the centrosome, which is necessary for centriole duplication and the fidelity of mitosis. — SMH

J. Cell Biol. 172, 505 (2006).

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