Report

Imaging Intracellular Fluorescent Proteins at Nanometer Resolution

Science  15 Sep 2006:
Vol. 313, Issue 5793, pp. 1642-1645
DOI: 10.1126/science.1127344

You are currently viewing the abstract.

View Full Text

Abstract

We introduce a method for optically imaging intracellular proteins at nanometer spatial resolution. Numerous sparse subsets of photoactivatable fluorescent protein molecules were activated, localized (to ∼2 to 25 nanometers), and then bleached. The aggregate position information from all subsets was then assembled into a superresolution image. We used this method—termed photoactivated localization microscopy—to image specific target proteins in thin sections of lysosomes and mitochondria; in fixed whole cells, we imaged vinculin at focal adhesions, actin within a lamellipodium, and the distribution of the retroviral protein Gag at the plasma membrane.

    View Full Text

    Cited By...