Science  15 Dec 2006:
Vol. 314, Issue 5806, pp. 1681
DOI: 10.1126/science.314.5806.1681b

We wish to retract our Report “Dual signaling regulated by calcyon, a D1 dopamine receptor interacting protein” (1). In this Report, enhanced D1 dopamine receptor (D1R)-stimulated intracellular Ca2+ release was attributed to a direct interaction with calcyon, a novel protein isolated in a yeast two-hybrid screen (Y2H) using the D1R C-terminal tail as bait. Resequencing of the cDNA clone obtained in the Y2H screen resolved a particularly GC-rich region upstream of the calcyon start codon that had been misread before and indicated that the calcyon coding sequence is out of frame with the GAL4 activation domain. In view of this, we conducted other in vitro studies and found that calcyon and D1Rs do not directly interact. Further, we also determined that although D1Rs do stimulate intracellular Ca2+ release after priming of cells (1) or neurons with Gq-linked receptor agonists (2), calcyon does not significantly enhance this response. The ability of calcyon and D1Rs to co-immunoprecipitate when co-expressed in cells as reported (1) presumably stems from the association of both proteins with clathrin-coated vesicles (3, 4). Likewise, calcyon is coexpressed in a number of D1R-positive neurons in brain and, like D1Rs, is found in dendritic spines. Thus, the isolation of the calcyon clone in a Y2H screen with D1Rs appears to have been adventitious. We must therefore retract this Report. We sincerely regret that we did not discover these errors before publishing. The third author of the Report, Steven Eubanks, has graduated from Medical College of Georgia and could not be located to sign the Retraction. The fifth author, Patricia Goldman-Rakic, is now deceased.

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