Research Article

Genes Required for Mitotic Spindle Assembly in Drosophila S2 Cells

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Science  20 Apr 2007:
Vol. 316, Issue 5823, pp. 417-421
DOI: 10.1126/science.1141314

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The formation of a metaphase spindle, a bipolar microtubule array with centrally aligned chromosomes, is a prerequisite for the faithful segregation of a cell's genetic material. Using a full-genome RNA interference screen of Drosophila S2 cells, we identified about 200 genes that contribute to spindle assembly, more than half of which were unexpected. The screen, in combination with a variety of secondary assays, led to new insights into how spindle microtubules are generated; how centrosomes are positioned; and how centrioles, centrosomes, and kinetochores are assembled.

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