Report

Sensing X Chromosome Pairs Before X Inactivation via a Novel X-Pairing Region of the Xic

+ See all authors and affiliations

Science  07 Dec 2007:
Vol. 318, Issue 5856, pp. 1632-1636
DOI: 10.1126/science.1149420

You are currently viewing the abstract.

View Full Text

Abstract

Mammalian dosage compensation involves silencing of one of the two X chromosomes in females and is controlled by the X-inactivation center (Xic). The Xic, which includes Xist and its antisense transcription unit Tsix/Xite, somehow senses the number of X chromosomes and triggers Xist up-regulation from one of the two X chromosomes in females. We found that a segment of the mouse Xic lying several hundred kilobases upstream of Xist brings the two Xics together before the onset of X inactivation. This region can autonomously drive Xic trans-interactions even as an ectopic single-copy transgene. Its introduction into male embryonic stem cells is strongly selected against, consistent with a possible role in trans-activating Xist. We propose that homologous associations driven by this novel X-pairing region (Xpr) of the Xic enable a cell to sense that more than one X chromosome is present and coordinate reciprocal Xist/Tsix expression.

View Full Text