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Science  08 Feb 2008:
Vol. 319, Issue 5864, pp. 829
DOI: 10.1126/science.319.5864.829

Multi-Application Imaging and Analysis

The G:BOX Chemi XT16 automated chemiluminescence and fluorescence imaging and analysis system features many innovations. Inside a newly designed light-tight darkroom, the latest 16-bit camera with a new f 0.95 variable aperture lens makes it possible to quickly produce accurate images of large gels and blots. The G:BOX Chemi XT16 offers computer control of its motor-driven stage, which allows the system to memorize set positions for specific applications. The camera, with sensitive effective 6.3-megapixel resolution, is ultracooled to guarantee the instrument can separate close band and spot images with virtually no background noise, even when imaging chemiluminescence blots for long exposure times. In addition, the darkroom comes with a state-of-the-art overhead white epi-lighting module, an ultraviolet to visible light NovaGlo converter screen, and a 20-cm-by-20-cm ultraviolet transilluminator to allow rapid and accurate imaging of common DNA and protein stains such as ethidium bromide, SYBR Green, SYBR Safe, Coomassie blue, Deep Purple, Pro-Q Diamond, silver stain, and SYPRO Ruby. For additional applications, it can be fitted with other lighting options, precision filters, and a blue light converter screen.


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Tissue Section Scanning

The Acumen eX3 is well suited to scanning tissue sections mounted on microscope slides due to its large field of view (400 mm2), which enables the rapid collection of multicolored fluorescence data from whole sections of tissue without the need to stitch together multiple images. The Acumen eX3 provides several modes of use. Cytometry mode rapidly scans, analyzes, and reports high-content information yielding very small postscan file sizes (>50kb). For more detailed evaluations, TIFF files can be exported and analyzed in batch mode using third-party image processing software. Cytometry mode can also be used to rapidly determine regions of interest within larger sections for visual scoring.

TTP LabTech

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3D Chemical Imaging with AFM

The alpha500 and alpha700 microscopy series are for automated confocal Raman imaging and atomic force microscopy (AFM) on large samples. The alpha500 and alpha700 are the first instruments on the market to combine confocal Raman microscopy for three-dimensional chemical imaging and AFM for high-resolution structural imaging in an automated system. A motorized sample stage with a travel range of 150 by 100 mm for the alpha500 and 350 by 300 mm for the alpha700 allows multiarea/multipoint measurements or overview scans on a user-defined number of measurement points. Automated functions such as integrated autofocus and an automatic AFM-tip approach guarantee that standardized routine measurement procedures or manually designed sequences can be performed without any ongoing process control by an operator. The instruments significantly reduce the overall experiment duration and deliver a greater amount of data in a given time to minimize resource use in routine research and deliver high-level quality control.


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High-Speed Imaging for Live Cell Research

Speed is often the deciding factor for successful imaging in high-resolution documentation of living cells, molecular processes, and rapidly fading fluorescence specimens. The monochrome digital camera Leica DFC360 FX is designed to produce brilliant images at maximum temporal resolution. Thanks to state-of-the-art charge-coupled device technology, the new camera system achieves frame rates of 20 fps for full frame and more than 100 fps in binning mode. The sensitive sensor and active Peltier cooling ensure a high dynamic range even for low-light intensities. With shutter speeds of 4 μs to 10 minutes and up to 10-fold signal amplification, the instrument offers maximum flexibility.


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Ultraviolet Area Imaging Detector

The ActiPix D100 is a miniature quantitative ultraviolet area imaging detector. It opens possibilities not available with conventional detectors, including new measurement capabilities in applications including real-time study of diffusion processes, inline quantification and sizing of biopharmaceuticals, dissolution and solubility testing, and membrane transport studies. The ActiPix D100 consists of a control box connected via a fiber optic cable and communications cable to a remote sensor head. The sensor head holds easily exchangeable, application-specific cartridges for techniques including capillary electrophoresis, nanoliquid chromatography, and imaging of lab-on-a-chip devices. The miniature detector head contains a high-resolution 1,280-by-1,024 active pixel sensor. The detector can be used as a plug-and-play accessory linked to multiple peripheral devices, such as capillary electrophoresis or nanoliquid chromatography instrumentation, with or without a mass spectrometer. Detection is performed at a selected wavelength by means of interchangeable filters. Processed data, including absorbance values covering the whole imaged area, is output in real-time to a computer using a high-speed serial data link.


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