The Chemical Genomic Portrait of Yeast: Uncovering a Phenotype for All Genes

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Science  18 Apr 2008:
Vol. 320, Issue 5874, pp. 362-365
DOI: 10.1126/science.1150021

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Genetics aims to understand the relation between genotype and phenotype. However, because complete deletion of most yeast genes (∼80%) has no obvious phenotypic consequence in rich medium, it is difficult to study their functions. To uncover phenotypes for this nonessential fraction of the genome, we performed 1144 chemical genomic assays on the yeast whole-genome heterozygous and homozygous deletion collections and quantified the growth fitness of each deletion strain in the presence of chemical or environmental stress conditions. We found that 97% of gene deletions exhibited a measurable growth phenotype, suggesting that nearly all genes are essential for optimal growth in at least one condition.

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