Signaling in Space

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Science  20 Jun 2008:
Vol. 320, Issue 5883, pp. 1565
DOI: 10.1126/science.320.5883.1565c

The surfaces where T cells interact with antigen-presenting cells have a distinct spatial organization of membrane components known as the immunological synapse. Shen et al. have devised a way to control the spatial presentation of antibodies to the T cell receptor (anti-CD3) and antibodies to the costimulatory receptor CD28 (anti-CD28). They used microcontact printing to create a surface in which either anti-CD3 and anti-CD28 were presented together or anti-CD28 was segregated in dots around anti-CD3 regions. T cells exposed to the surface localized to the antibody-coated regions of the surface regardless of antibody segregation, but secretion of interleukin 2 was increased if anti-CD28 was segregated at the periphery of a central locus of anti-CD3 rather than uniformly distributed with it. Activation of the kinase Akt was also greater in cells exposed to the segregated signals. The dynamics of cell interaction with the receptors revealed that cells transiently contacted and released patches containing segregated anti-CD28 but remained associated with surfaces where the ligands were mixed. This system may foster insight into how the geometry of immunological synapses influences intracellular signaling. — LBR

Proc. Natl. Acad. Sci. U. S. A. 105, 7791 (2008).

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