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Science  16 Jan 2009:
Vol. 323, Issue 5912, pp. 405
DOI: 10.1126/science.323.5912.405a

DNA Amplification from Whole Blood

The Phusion Blood Direct PCR Kit for amplification of DNA from whole blood eliminates the need for DNA purification prior to polymerase chain reaction. The kit makes use of a modified Phusion Hot Start DNA Polymerase, which is highly resistant to the inhibitors in blood and retains its activity even at 40 percent blood concentration. The kit allows analysis of blood samples with different anticoagulants, under different storage conditions, and from various species. It tolerates heparin, citrate, and EDTA, and is compatible with blood stored on various types of filter paper.

Finnzymes

For information +358-9-2472-3291

http://www.finnzymes.com/

Genomic Sequencing System

The RDT 1000 incorporates microdroplet-based technology to amplify hundreds to thousands of genomic loci with high specificity and uniformity. It leverages the sensitivity and specificity of the polymerase chain reaction while avoiding the limitations of alternative multiplex hybridization and amplification techniques. Targeted sequencing is one of the fastest growing applications for next-generation sequencing technology. Sequence enrichment is a critical step in an efficient targeted sequencing workflow. The system allows researchers to isolate specific regions of the genome for subsequent sequencing. The system can be used for simple, fast, walkaway workflow of DNA sample preparation, reagent delivery, on-chip processing, and sample recovery.

RainDance Technologies

For information 888-724-6440

http://www.raindancetechnologies.com/

Nucleic Acid Extractor

The SPRI-TE Nucleic Acid Extractor for the automated purification of DNA and RNA prepares up to 10 samples in about 30 minutes and requires minimal user training. Prepackaged, ready-to-use extraction kits are available for preparing genomic DNA, viral nucleic acid, or formalin-fixed, paraffin-embedded nucleic acid samples. The system features proprietary SPRI (solid phase reversible immobilization) technology, which extracts the nucleic acids without centrifugation or a vacuum manifold. This technology ensures stronger binding and higher quality purification than silica-coated beads, which can shear nucleic acids, lowering the quality of results.

Beckman Coulter

For information 714-993-8955

http://www.beckmancoulter.com/

PCR Inhibitor Removal Kits

The OneStep and OneStep-96 PCR Inhibitor Removal Kits contain all the components necessary to remove from DNA and RNA preparations contaminants that can inhibit downstream enzymatic reactions such as polymerase chain reaction (PCR) and reverse transcription (RT). The column and plate matrices are designed to remove polyphenolic compounds, humic and fulvic acids, tannins, melanin, and more. In most commercially available kits, these inhibitory compounds can contaminate impure DNA and RNA preparations isolated from soil, plants, skin, fecal, and other samples. Sample cleanup is as simple as applying, spinning, and recovering a sample from the column or plate.

Zymo Research Corporation

For information 888-882-9682

http://www.zymoresearch.com/

DNA and RNA from Same Soil Sample

The RNA PowerSoil DNA Elution Accessory Kit is designed to coisolate high-molecular-weight DNA for the recovery of the total nucleic acid content of the original sample. It can be used in combination with the RNA PowerSoil Total RNA Isolation Kit to isolate and elute the RNA and DNA into separate fractions. The kits include patented new technology that removes all known polymerase chain reaction inhibitors, including humic acids, polysaccharides, polyphenolics, melanin, and hematin.

Mo Bio Laboratories

For information 760-929-9911

http://www.mobio.com/rnapowersoildna

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Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizations are featured in this space. Emphasis is given to purpose, chief characteristics, and availabilty of products and materials. Endorsement by Science or AAAS of any products or materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier.

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