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Science  17 Apr 2009:
Vol. 324, Issue 5925, pp. 411
DOI: 10.1126/science.324.5925.411a

Nanoscale Liquid Chromatography

Designed to bring improved reproducibility and ease of use to proteomics research, the cHiPLC-nanoflex System is a docking station for up to three microfluidic chips that contain NanoLC columns or trap columns. The proprietary EksPort microconnector system ensures consistent fluidic connections to facilitate column changes. Chips can be interchanged in seconds, even by inexperienced users, with minimal dead volume to compromise resolution. The proprietary microfabrication process used for both the trap chips and analytical column chips delivers exceptional column-to-column reproducibility. Superior quality separations and reproducibility are critical to the success of biomarker validation studies. In these applications, in which peptides are measured by multiple reaction monitoring, the improved column-to-column reproducibility delivered by the cHiPLC-nanoflex helps in obtaining consistent data from large numbers of samples.

Eksigent

For information 925-560-2600

http://www.eksigent.com/

Dry Blotting System

The iBlot Dry Blotting System for protein immunoblotting offers complete protein transfer in 7 minutes or less. It offers reduced variability in blot preparation and running and high-quality transfer without bubbles or distortions. It features the convenience of a self-contained unit, with no need for added buffers or an external power supply.

Invitrogen

For information 800-955-6288

http://www.invitrogen.com/

Protein Detection Kits

The Kodak X-Sight Western Kits offer an analytical technique to detect proteins. The kits are built on X-Sight Nanosphere Conjugates, which are available in four distinct wavelengths, including three in the near-infrared range. The kits are offered in several different wavelength combinations that include up to three conjugates, making them suitable for multiplex applications. Each kit includes membranes and buffers to achieve high signal-to-noise ratios.

Carestream Health

For information 877-747-4357

http://www.carestreamhealth.com/

Microwave Peptide Cleavage System

The Accent Cleavage System is a microwave peptide cleavage system that enables chemists to perform a full peptide cleavage in less than 30 minutes and microcleavages in as little as 2 minutes. The rapid microcleavage enables chemists to get a liquid chromatography/mass spectrometry analysis of their peptide in as little as 15 minutes, so they can quickly determine how their synthesis is progressing. The system is fast, improves peptide purities and yields, and enables chemists to use less noisome reagents than other cleavage systems. The system accepts 4-ml, 25-ml, and 35-ml vessels and a cleavage scale of microcleavage to 0.25 mmol.

CEM Corp.

For information 704-821-7015

http://www.cem.com/

Protein Interaction System

The DUALhunter System is a flexible assay system designed to find novel protein interaction partners for transcriptionally active and self-activating proteins. The system quickly screens full-length soluble nuclear proteins in vivo and can also be used to screen protein domains or fragments for novel protein interactions. It also permits easy subcloning of full-length complementary DNA (cDNA) inserts; the protein of interest is screened against a cDNA library of choice, which can be very large, and cell-type specific interactions can be detected. The system detects protein interactions based on the split-ubiquitin system, giving researchers the ability to screen classes of proteins such as transcription factors or strongly acid proteins that cannot be screened using classic yeast two-hybrid systems.

Dualsystems Biotech

For information +41-(0)1-738-50-00

http://www.dualsystems.com/

IgG Antibody Purification

BioVyon Protein A microplates and columns offer a simple and rapid method for antibody purification. The new products give a consistent recovery of immunoglobulin G (IgG) antibody that is independent of the flow rate during loading. Unlike existing bead-based technologies, there are no time-consuming slurries to equilibrate and pour into columns. BioVyon Protein A Mini Columns and Micro Columns give consistent recoveries of purified IgG. The high purity is consistent in multiple extractions using the same column or from column to column. The columns can be reused several times. The minicolumns have a capacity of 2.5 mg IgG, and the microcolumns have a 10 μg capacity.

Porvair Filtration Group

For information +44-1489-864330

http://www.biovyon.com/

Protein Extraction Buffers

The Mammalian Protein Extraction Buffer and Yeast Protein Extraction Buffer Kit are mild, nondenaturing buffer compositions that deliver high-quality protein lysate compatible with most downstream applications without the need for mechanical cell lysis. Both products give highly reproducible, consistent results that maintain biological activity. The mammalian buffer is designed for use in cultured cells, and can be used for both cell suspensions and adherent cells. The yeast kit is suitable for preparation of approximately 10 ml of yeast cell pellet suspension and eliminates the need for glass beads. The buffers are compatible with downstream applications such as chromatography, enzyme assays, and electrophoresis.

GE Healthcare

For information 262-501-0777

http://www.gelifesciences.com/

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Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizations are featured in this space. Emphasis is given to purpose, chief characteristics, and availabilty of products and materials. Endorsement by Science or AAAS of any products or materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier.

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