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Science  31 Jul 2009:
Vol. 325, Issue 5940, pp. 629
DOI: 10.1126/science.325_629a

Confocal FRAP Imaging

The VT-HAWK combines high precision point-scanning fluorescence recovery after photobleaching (FRAP) with multibeam confocal imaging. The instrument's ability to switch between imaging and photo-bleach modes in seconds enables accurate quantitation of fluorescence intensity dynamics in precise, user-defined regions of the image. VT-HAWK supports FRAP, fluorescence loss in photobleaching, photo-activation, and photo-conversion techniques with selectable pinhole sizes for confocal imaging. The instrument's optics deliver optimal laser beam quality at the sample without compromising the performance of either mode. Options include a laser-merge module that houses up to six solid-state lasers.

Interferon Kit

The VeriKine-HST Human Interferon-Beta Serum ELISA Kit is an enzyme-linked immunosorbent assay for measuring human interferon-beta in serum, plasma, and cell culture supernatant samples. It offers high sensitivity, detecting levels as low as 0.5 IU/ml, with an excellent coefficient of variation. It is not affected substantially by the presence of sample contaminants.

Electroelution of Nucleic Acids and Proteins

G-Capsule is a simple electroelution device that excises DNA or protein bands from agarose and polyacrylamide gels and elutes sample in a final volume of 30 µl. Gentle electroelution through G-Capsule eliminates the risks of damage to DNA or protein samples commonly encountered in the use of glass milk, spin columns, binding columns, and other popular techniques. The simple method eliminates washing, spinning, heating, and precipitation steps. The kit has two parts, G-Pick and G-Trap. The user picks up the protein or nucleic acid band with the G-Pick and assembles it with the G-Trap. The assembled G-Capsule is submerged in electrophoresis buffer on a horizontal electrophoresis system and the protein or nucleic acid is rapidly eluted into the G-Trap. The recovered DNA can be used in molecular manipulations such as ligation, restriction enzyme digestion, sequencing, amplification, random priming, and other enzymatic reactions.

Magnetic Stirrers

A new line of magnetic stirrers comes in three sizes and four colors. They are available in sizes 120 mm, 170 mm, and 220 mm, with stirring capacities of 2 liters, 4 liters, and 5 liters, respectively. Stirring speed range is from 150 rpm to 2,500 rpm. The tops are made of polypropylene and come with two nonslip silicon covers (colored black and white) to prevent sliding of the sample vessel. A wide base and rubber feet make these quiet stirrers extremely stable.

High Throughput Electrophoresis

The CSL multiSUB4 is a compact electrophoresis gel system capable of running four gels in one tank for optimum throughput while saving time and space. The system can run up to 1,200 samples in four vertically stacked gel trays to provide high throughput electrophoresis with the space-saving advantage of a small unit. Each gel tray is ultraviolet-transparent to ensure easy visualization of samples directly on the transilluminator following electrophoresis. The inclusion of four double-sided, multichannel-pipette-compatible combs allows rapid sample loading from 96-well gel trays and thermal cycler blocks. The multiSUB system is available with two formats of double-sided combs, one with one marker and one sample lane for the large scale-up of nucleic acids that may be turned over for a 12-sample throughput, and another included with the unit that offers both eight-sample and 18-sample throughput.

Cell Culture Inserts

The Hanging Millicell Cell Culture Inserts are available preloaded into 24-well receiver trays to save time and labor in a range of experimental protocols. Millicell inserts are plastic cell culture wells with membrane bottoms designed to promote more natural cell behavior than plastic plates. The porous membrane allows cells to be fed from both the apical and basolateral sides. As a result, the cells form high-integrity membranes, and studies have shown that cell growth and function more closely resemble those of cells grown in vivo. The hanging, preloaded inserts are available with a polyethylene terephthalate (PET) membrane in two common pore sizes (0.4 and 8.0 micron). The insert's unique design allows easier basolateral access than other hanging inserts with less risk of contamination. The units are sterile and tissue-culture treated. The hanging inserts are recommended for coculture and permeability assays including angiogenesis, chemotaxis, invasion, feeder layers, immunohistochemistry, tumor cell metastasis, and invasion assays.

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