Technical Comments

Response to Comment on “The Dynamic Control of Kiss-And-Run and Vesicular Reuse Probed with Single Nanoparticles”

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Science  18 Sep 2009:
Vol. 325, Issue 5947, pp. 1499
DOI: 10.1126/science.1176007

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Abstract

Granseth et al. argue that vesicle retrieval at hippocampal synapses is fully accounted for by a single mode of endocytosis. However, their assay focused on readily releasable pool vesicles (RRP), not RRP + reserve pool vesicles in tandem, and therefore cannot detect pool-dependent changes in vesicle-retrieval kinetics during a stimulus train. Using a probe similar to theirs, we observed rapid vesicle retrieval consistent with Kiss-And-Run fusion.

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