Report

Creating Bacterial Strains from Genomes That Have Been Cloned and Engineered in Yeast

Science  25 Sep 2009:
Vol. 325, Issue 5948, pp. 1693-1696
DOI: 10.1126/science.1173759

You are currently viewing the abstract.

View Full Text
As a service to the community, AAAS/Science has made this article free with registration.

Abstract

We recently reported the chemical synthesis, assembly, and cloning of a bacterial genome in yeast. To produce a synthetic cell, the genome must be transferred from yeast to a receptive cytoplasm. Here we describe methods to accomplish this. We cloned a Mycoplasma mycoides genome as a yeast centromeric plasmid and then transplanted it into Mycoplasma capricolum to produce a viable M. mycoides cell. While in yeast, the genome was altered by using yeast genetic systems and then transplanted to produce a new strain of M. mycoides. These methods allow the construction of strains that could not be produced with genetic tools available for this bacterium.

  • * Present address: Biotechnology Industry Organization (BIO), 1201 Maryland Avenue SW, Washington, DC 20024, USA.

View Full Text

Cited By...