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Science  19 Feb 2010:
Vol. 327, Issue 5968, pp. 1026
DOI: 10.1126/science.327.5968.1026-a

Fluidic Heating Control

The Mitos Hotplate Adapter enables exact temperature control of microfluidic chips in conjunction with a range of hotplates. It is compatible with the Mitos Micromixer chip, the Mitos T-junction chip, the Mitos Thin-layer chip, and the Mitos Droplet Generator chip. With a compact footprint of 39 mm by 43 mm, the Hotplate Adapter provides precise control over reaction temperatures. Its excellent chemical compatibility, along with quick-chip connection and release, makes it easy to use in heat-controlled microfluidic applications. The Mitos Hotplate provides a temperature range of room temperature to 300°C, facilitating a wide range of processes. This hotplate is digitally controlled with an external thermocouple temperature probe, which can be attached to the Hotplate Adapter for optimal temperature control.

High-Volume Microplate

The Krystal 24 microplate's 3.1-ml well volume makes it suitable for screening applications that require high sensitivity combined with photometrics accuracy and repeatability. The large surface area of the wells makes the tissue-culture-treated version of the plate suitable for cell-growth applications. The Krystal 24 microplates are constructed of ultrapure-grade polystyrene and available in opaque white and solid black formats. The black plate provides the all-absorbing background needed to minimize background interference for sensitive fluorescence measurements. The opaque white plate maximizes reflectivity, enabling even weakly emitting luminescence assays to be routinely undertaken. In addition to optimized luminescence and fluorescence measurements, the unique design offers improved cell-binding efficiency and provides the convenience of direct measurement on bottom-reading spectrophotometers and inverted microscopes.

Microfluidic Glass Chip

The glass Mitos Capillary Electrophoresis Chip A is designed to separate small quantities of biological molecules by capillary electrophoresis. Measuring only 15 mm by 45 mm by 2 mm, this electrophoresis chip combines a cross-channel design with a 20-μm channel depth and a 30-mm long channel for accurate analysis of the separation. Offering excellent chemical compatibility, the chip separates species in the interior of the microchannel based on their size-to-charge ratio. The high surface-to-volume ratio of the microchannels enables the application of high voltages without overheating the samples. An extremely smooth channel surface, in combination with a wide pressure and temperature range, makes it suitable for a broad range of applications.

Assay Panels

The Milliplex MAP EpiQuant assay panels are a new family of phosphotyrosine multiplex assays designed to advance cell signaling research. Compared with existing techniques, these assays give researchers a more complete view of how cells send molecular signals. The Milliplex MAP EpiQuant platform can quantify numerous signals coming from either a single protein, multiple proteins in a pathway, or multiple pathways. The assays allow the simultaneous measurement of more than 40 analytes in one well, so whole pathways can be analyzed in a single 96-well plate. Researchers can also use the panels to detect cross-talk between pathways. The assays provide absolute quantitation of total and phosphorylated proteins, enabling direct comparison of signaling events in different pathways.

Cell Migration Assay

The Oris Pro Cell Migration Assay makes use of a nontoxic biocompatible gel to form a cell-free zone on cell culture surfaces. After cells are seeded into the 96-well plate, the gel dissolves, permitting cells to migrate into the well centers. The kit enables researchers to save time and money by using automated liquid-handling equipment for fast setup of high throughput assays. Researchers can capture and quantify real-time cell migration data using microscopes and high content screening, and high content imaging instruments. The new Oris Pro Assay complements the Oris line of cell migration assay products that feature patent-pending, cell-seeding stoppers to create cell-free detection zones in the center of each well.

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  • Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizations are featured in this space. Emphasis is given to purpose, chief characteristics, and availabilty of products and materials. Endorsement by Science or AAAS of any products or materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier.

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