Cell Biology

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Science  19 Feb 2010:
Vol. 327, Issue 5968, pp. 925
DOI: 10.1126/science.327.5968.925-b

Within the nucleus, chromatin is spatially organized, and the intranuclear locations of genes can be correlated with their transcriptional activity. Ahmed et al. have identified two DNA sequences in chromatin that target genomic loci to the nuclear envelope in yeast. Peripheral targeting of genes, particularly those activated by stresses such as heat shock or nutrient deprivation, such as INO1, is linked to active transcription. Using deletion analyses and engineered plasmids, the authors identified an eight-base sequence in the promoter of INO1, that when mutated, inhibited targeting of ectopic INO1 to the nuclear periphery, as well as its transcriptional activation. They also identified several protein components of the nuclear pore complex (the exit channels from the nucleus to the cytoplasm) that were required for this localization. For peripheral targeting of the endogenous INO1 gene, a second sequence in an upstream gene was also required. Searching through the genome, the authors identified 94 promoters that contained the eight-base targeting sequence, many of which were activated by heat shock, suggesting a common mechanism of activation and peripheral localization.

Nat. Cell Biol. 12, 111 (2010).

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