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Science  12 Mar 2010:
Vol. 327, Issue 5971, pp. 1395
DOI: 10.1126/science.327.5971.1395-a

TaqMan Pri-MIRNA Assays

The TaqMan Pri-miRNA Assays are a set of real-time polymerase chain reaction (PCR) assays that enable researchers to measure the activity of specific microRNA (miRNA) genes by detecting and quantifying levels of expressed primary miRNA transcripts. Based on the latest Sanger miRBase content, this new line consists of more than 1,200 primary miRNA assays for human, mouse, and rat that have been designed using a stringent bioinformatic pipeline and the TaqMan Assay chemistry. Each assay delivers the specificity and sensitivity necessary to accurately measure primary miRNA transcript expression levels from a single genomic locus, without the requirement for a fully defined primary transcript sequence.

Quantitative Real-Time Analysis

The DyNAmo ColorFlash qPCR (quantitative polymerase chain reaction) Kits for fast, real-time analysis provide detection and quantification of DNA sequences from various sources. The kits are designed to ease qPCR setup and minimize errors by producing a simple way to track pipetting of reaction components. The two kits, DyNAmo ColorFlash SYBR Green qPCR Kit and DyNAmo ColorFlash Probe qPCR Kit, feature an innovative multicolor system. The Master Mix contains a blue dye, and a separate sample buffer contains a yellow dye. The qPCR reactions mix containing both components is green. With this patent-pending multicolor system, pipetting of both the master mix and the sample can be easily monitored, decreasing the risk of errors.

Melting Analysis Kits

Epigenetics researchers can now detect changes in methylation status in real-time using high-resolution melting (HRM) analysis. The EpiTect HRM polymerase chain reaction (PCR) kit provides a master mix format for the detection of changes in the CpG (C-phosphate-G) methylation status of bisulfite-converted DNA. The kit is designed to run on all real-time PCR cyclers, including the Rotor-Gene Q, which provides a highly specific melting curve. HRM technology enables a rapid characterization of DNA samples based on their melting behavior following PCR amplification, and is suitable for screening large numbers of samples and for detecting changes in the methylation degree of specific DNA regions.

RT-PCR Kits

The Quantifast Multiplex RT-PCR and Rotor-Gene Multiplex RT-PCR kits are new reverse transcription–polymerase chain reaction (RT-PCR) tools. The Quantifast kits perform fast real-time RT-PCR without requiring optimization on both standard and fast real-time cyclers for one-step applications. The Rotor-Gene Kit allows detection of up to four targets per tube and includes optimized protocols for precise results on the Rotor-Gene Q real-time cycler.

Adjustable Spacing Pipettor

The six-channel Voyager electronic pipettor features a mechanism that alters tip spacing at the press of a button. The Voyager incorporates a small motor that moves the center-to-center tip spacing quickly and smoothly at any point in the protocol, and requires only one hand to operate. Users can preset up to three distinct tip spacings in a way that accommodates most labware vessels. The spacing can be adjusted from 9.0 mm to 19.5 mm. Two models are available, in volume ranges of 10–300 μl and 50–1,250 μl. Both models work with 24-well plates, 48-well plates, and many other formats.

Sample Quantification System

SlingShot is a DNA quantification system that allows researchers to sequence their libraries of rare samples and lower costs, improve data quality, and speed the time to results in DNA sequencing. It also allows the sequencing of rare samples. It can improve the productivity of next generation sequencing tools from Roche, Illumina, and Applied Biosystems by exploiting the unique microfluidic properties of integrated fluidic circuits (IFCs) to detect only amplifiable molecules within the sample mixture. IFCs use extremely small amounts of sample, so this technology opens up the ability to sequence rare libraries where suboptimal amounts of the tissue are available. The ability of IFCs to count individual molecules—using digital polymerase chain reaction—also eliminates the need for costly library titration to ready samples for sequencing.

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  • Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizations are featured in this space. Emphasis is given to purpose, chief characteristics, and availabilty of products and materials. Endorsement by Science or AAAS of any products or materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier.

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