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Science  16 Apr 2010:
Vol. 328, Issue 5976, pp. 380
DOI: 10.1126/science.328.5976.380-a

FLUORESCENCE SPECTROMETER

The Lumina is a research-grade, dual-monochromator scanning fluorescence spectrometer that offers a new level of clarity in fluorescence measurement. Designed for both demanding research applications and routine laboratory analyses, the Lumina delivers 0.5 nm spectral bandwidth, high sensitivity, and fast scanning speed. Its ability to provide 0.5 nm spectral bandwidth gives it superior resolving power compared with competitive systems in the same price range. It offers lower limits of detection, less noise, and more consistent baseline measurements. Its powerful xenon lamp, combined with optimized monochromators, allows for the detection of sample concentrations below the parts-per-billion level for heavy metals, polycyclic aromatic hydrocarbons, and more. Its photomultiplier detector offers extended measurement of the near-infrared wavelength, for cutting-edge research in biochemistry and photosynthesis applications.

FLUORESCENCE MICROSCOPES TOOL

The VivaTome for fluorescence microscopes is a tool for developmental and cell biologists to examine the dynamics of living specimens without extensive prior knowledge of optical sectioning. The VivaTome offers ease of use and excellent results in the visualization of fast processes. It is designed for applications in which temporal resolution is a priority. It provides clear, easy-to-interpret and quantifiable results for biological specimens, whether cell structures, tissue sections, or living organisms. Using simple and economical white light illumination, it can achieve frame rates of up to 30 frames a second. It can be used with the entire spectrum of Zeiss objective lenses with consistently high optical section quality.

FLUORESCENT PROTEIN AND TECHNOLOGY CATALOG

The 2010 Evrogen Catalog describes innovative novel fluorescent proteins for biological research and commercial biotechnology applications. The catalog includes basic Tag and Turbo fluorescent proteins, photoactivatable proteins, fluorescent biosensors, and antibodies to detect fluorescent proteins, as well as a panel of nucleic acid research kits. It describes fluorescent proteins ranging from blue to far-red, covering a spectrum of eight colors useful for multicolor labeling of cells, cell organelles, and proteins. It includes a pair of fast-maturing far-red fluorescent proteins for whole body imaging and a set of fluorescent proteins that can be used in fluorescence resonance energy transfer applications as donors and acceptors.

MICROPLATE READER

The Biochrom Asys UVM 340 is a monochromator-based plate reader that lets the user select the exact wavelength to measure optical density for an application, without compromise. The user can select to measure absorbance at any wavelength between 340 nm and 800 nm at 1 nm intervals. The instrument is suitable for enzyme-linked immunosorbent assays and other absorbance applications with no need to purchase expensive extra filters or to change instruments to perform different assays. The Biochrom Asys UVM 340 is a single-channel microplate reader that can read 12 96-well plates, for greater plate-format flexibility than multichannel systems. It can be integrated into automated systems and features a compact footprint.

FLUORESCENT CONJUGATES

Several new DyLight fluorescent conjugates for immunofluorescent staining are available for conjugation to affinity-purified antimouse IgG, antirabbit IgG, and streptavidin. The Dylight fluorescent dyes are direct alternatives to traditional fluorophores such as fluorescein and rhodamine. The dyes currently offered are DyLight 488 (green), Dylight 549 (orange), and DyLight 594 (red). These new conjugates offer brighter fluorescence and greater photostability than other fluorochromes, yet can be used in existing protocols because of their parallel excitation/emission spectra. They are stable over a pH range of 4 to 9. They can be used as single labels or in combination with other fluorophores.

NEAR INFRARED FLUORESCENT DYES

The DyLight 680B Dyes for directly labeling proteins and other biomolecules exhibit fluorescence intensity up to 10 times greater than similar dyes. Their applications include enzyme-linked immunosorbent assays, fluorescence microscopy, protein arrays, in vivo imaging, and protein immunoblot analyses. They are available in both amine-reactive and sulfhydryl-reactive chemistries. They exhibit excitation and emission spectra at 679 nm and 698 nm, respectively.

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  • Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizations are featured in this space. Emphasis is given to purpose, chief characteristics, and availabilty of products and materials. Endorsement by Science or AAAS of any products or materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier.

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