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Science  24 Sep 2010:
Vol. 329, Issue 5999, pp. 1676
DOI: 10.1126/science.329.5999.1676-a


A range of compact, space-saving bench top incubators, or hybridization units, provide a chamber temperature from ambient to 85°and are ideal for many laboratory tasks, such as cell and tissue culture applications. The range encompasses 21 models which all feature microprocessor control of temperature in precise 0.1°increments as well as continuous and programmable timer function which is easily viewed within a 2.6 inch LCD screen. Advanced ventilation design technology maintains accuracy to within ±° The standard incubator model is supplied with stainless steel mesh shelves, while the more sophisticated options are available with specialist vortex, orbital, rocking, or reciprocal shaking platforms. They are compact and stackable, requiring only a small footprint ensuring maximum space-saving and multitasking.

Cell Culture Devices

The PetakaG3 Cell Culture Device reduces cell culture contamination, improves portability, saves space, and cuts costs for a wide range of biotechnology and medical purposes. Unlike conventional culture devices, PetakaG3 is Virtually Hermetically Enclosed—requiring no additional CO2 or additional humidity, effectively eliminating contamination, spills, and leaks. As a result, live cells can be handled safely and shipped in PetakaG3 devices without requiring freezing or dry ice. Its compact size permits up to fifteen times more cell culture surface, per incubator. The innovative design is based on a resealable access port that keeps the interior of the device hermetic to exterior elements. Combined with a gas transfer quenching system and filtered exit vent, this permits safe and easy one-step access or retrieval. There are no caps or openings, no atmospheric contamination and no saturated humidity or added CO2 needed in the incubator.

Disposable Bioreactor system

The CellMaker PLUS is a state-of-the-art and easy-to-use disposable bioreactor system specifically designed for efficient growth of a wide range of cell types, including E.coli, pichia, algae, and bacteriophage, as well as the more challenging mammalian and insect cells. This single-use bioreactor system is available with both 8 L and 50 L capacities to provide flexibility in scale. It includes a patented air dispersion system to effectively aerate and mix the cell culture and features a modular control system which is flexible and adaptable, and can be configured to satisfy the needs of the application. This system is simple, scalable, and inexpensive to use. The CellMaker PLUS controller allows for precise management of temperature (from 15°to 45°, controls the flow and mix of sparge gas, and enables monitoring and control of pH and dissolved oxygen.

Epithelial cell culture

EpiGRO epithelial cells and culture media products have been optimized to provide improved cell culture for critical research applications. The family of EpiGRO cell types includes keratinocytes, small airway epithelial cells, corneal cells, and prostate epithelial cells. Each EpiGRO medium is uniquely formulated to provide an optimized environment for its corresponding EpiGRO cell type. Consequently, cells proliferate faster, exhibit improved morphology, and show better viability than epithelial cells cultured in other media formulations. The media are provided in unique, light-blocking, temperature-monitored packaging to ensure stability and protect the media from damage by light and contamination.

Customized Cell Lines

Stable cell lines that express specific proteins of interest along with a wide selection of tags are offered through a new customized service. The two part process takes just six weeks to complete. Available cell lines include: HEK293 (human embryonic kidney cells), INS-1 (beta pancreatic islet cells), CHO (Chinese hamster ovary cells), and 3T3 (primary mouse embryonic fibroblast cells), along with many others. The first part of the process consists of constructing an integration vector containing the gene of interest, which is then transfected into the appropriate cell line. Tags can be placed at either the N- or C-terminus. Once a stable cell line has been selected, clones are expanded, and expression of the protein is verified by Western blotting. Upon completion of the process, five vials of frozen stocks with at least five million cells per vial, a Western blot demonstrating protein expression, and the cell cultivation protocol are delivered.

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  • Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizations are featured in this space. Emphasis is given to purpose, chief characteristics, and availabilty of products and materials. Endorsement by Science or AAAS of any products or materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier.

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