Cell Biology

First In, Last Out

See allHide authors and affiliations

Science  22 Oct 2010:
Vol. 330, Issue 6003, pp. 428-429
DOI: 10.1126/science.330.6003.428-c

The mammalian cell nucleus is surrounded by a double membrane punctured by nuclear pores. The entire nuclear envelope is disassembled during mitosis and then reassembled in each daughter cell, and postmitotic assembly has been studied in some detail. Dultz and Ellenberg have asked what happens when additional pores are required. High-resolution light microscopy allowed the visualization of single nuclear pore complexes in living cells during interphase. As the nucleus grew, new pore complexes were assembled at a steady rate and involved the accumulation of one of the nuclear pore membrane proteins, Pom121, which then recruited other soluble pore components. This order of assembly is the opposite of that seen at the end of mitosis, which implies that the two processes are mechanistically distinct.

J. Cell Biol. 191, 15 (2010).

Navigate This Article