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Science  29 Oct 2010:
Vol. 330, Issue 6004, pp. 692
DOI: 10.1126/science.330.6004.692-a

Nanoliter Liquid Handling

The mosquito provides precise and repeatable nanoliter dispensing of any liquid down to 25 nL, irrespective of liquid viscosity. The liquid handler is available in three standard formats, each of which is flexible enough to address a range of applications, including high throughput screening (HTS), 'hit picking', and protein crystallography. Making protein crystallography exceptionally fast and cost effective, the mosquito Crystal automates crystallography setups (hanging drop, sitting drop, and microbatch techniques) and additive screening. Mosquito LCP offers an additional fully automated solution to lipidic cubic phase (LCP) screening. Mosquito HTS miniaturizes screening applications, such as assay-ready plate preparation, reformatting, and serial dilutions to make more efficient use of valuable compounds. Finally, mosquito X1 is a single tip 'hit picking' instrument, which offers precision nanoliter sampling of any individual well or location. This enables researchers to transfer nanoliter volumes of hits from primary screening plates directly to hit validation without cross-contamination or further dilution.

Protein Extraction

The Universal Protein Extraction (UPX) Kit is designed for unbiased, high-yield detergent extraction of proteins from cells and tissue. The kit extracts both soluble and insoluble proteins at high efficiency, enabling subsequent analysis of membrane proteins and other hydrophobic proteins. Designed to solubilize the whole proteome, including difficult membrane proteins, the UPX Kit gives researchers more protein from their tissues to work with and opens opportunities for researchers to analyze specific insoluble proteins, or to obtain a more encompassing understanding of the proteome. The UPX Kit provides high-yield, unbiased protein extraction results from mammalian tissue, mammalian-derived cell lines, bacteria, and other samples commonly used for protein research. Starting material can be frozen or freshly harvested.

Gene Delivery

A new range of expression-ready lentiviral particles allow easy gene delivery into hard-to-transfect cells, including primary cells, nondividing cells, and cells in suspension. The particles are supplied ready-to-use, eliminating the need for any lipids or transfection reagents. The range of premade lentiviral particles deliver genes in a highly reproducible and controllable manner for the expression of greater than 250 human proteins, mouse proteins, enzymes, and fluorescent markers. These particles can also be used to easily and cost-effectively create stable cell lines for long-term high constitutive or Tet inducible expression applications in the presence of TetR (a repressor protein). Further, lentiviral particles can also be used in vivo for the generation of transgenic animals and for inducing pluripotent stem cells. Custom lentiviral particles for the expression of your own gene of interest or shRNA can also be generated.

Protein Purification

The Glutathione Agarose family of products is designed for cost-effective purification of glutathione S-transferase (GST)-tagged proteins from cellular lysates. Pierce Glutathione Agarose consists of glutathione linked to agarose beads though a 12-atom spacer which minimizes steric hindrance effects during purification. The six percent beaded agarose and highly purified glutathione limit nonspecific binding and result in a high binding capacity of greater than 25 mg of GST per milliliter of settled resin. The purification resin can be reused at least five times without loss in functionality. The affinity purification of GST-tagged proteins using Pierce Glutathione Agarose is a simple, one-step process. Bound GST-tagged proteins are easily eluted with a buffer containing Thermo Scientific Reduced Glutathione. If the GST fusion protein contains a Factor Xa protease site, then the protein can be cleaved from the GST tag with Thermo Scientific Factor Xa. Pierce Glutathione Agarose is available in numerous formats and as a standalone resin.

Plasmid DNA Extraction

The GETPlasmid DNA kits are spin column purification kits for the isolation of high quality plasmid from bacterial cultures. These kits are available in different formats: Maxiprep, Mediprep, Miniprep, and a 96-well kit. The different formats allow you to isolate various yields of plasmid. The GET Plasmid kits use an extraction method based on alkaline lysis of bacteria and uses our high affinity GET Plasmid columns to bind and purify plasmid DNA. This quick and easy protocol eliminates toxic phenol/chloroform extractions or ethanol precipitations. The extracted plasmid is ready for further DNA manipulation such as restriction enzyme digestion, ligation, transformation, transfection, and sequencing.

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  • Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizations are featured in this space. Emphasis is given to purpose, chief characteristics, and availabilty of products and materials. Endorsement by Science or AAAS of any products or materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier.

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